Publications

@article{Shi2024,

title = {Long‐Term Autotrophic Growth and Solar‐to‐Chemical Conversion in \textit{Shewanella Oneidensis} MR‐1 through Light‐Driven Electron Transfer},

author = {Yan Shi and Kejing Zhang and Jianxin Chen and Bingtian Zhang and Xun Guan and Xin Wang and Tong Zhang and Han Song and Long Zou and Xiangfeng Duan and Haichun Gao and Zhang Lin},

doi = {10.1002/anie.202412072},

issn = {1521-3773},

year  = {2024},

date = {2024-12-16},

urldate = {2024-12-16},

journal = {Angew Chem Int Ed},

volume = {63},

number = {51},

publisher = {Wiley},

abstract = {<jats:title>Abstract</jats:title><jats:p>Members of the genus <jats:italic>Shewanella</jats:italic> are known for their versatile electron accepting routes, which allow them to couple decomposition of organic matter to reduction of various terminal electron acceptors for heterotrophic growth in diverse environments. Here, we report autotrophic growth of <jats:italic>Shewanella oneidensis</jats:italic> MR‐1 with photoelectrons provided by illuminated biogenic CdS nanoparticles. This hybrid system enables photosynthetic oscillatory acetate production from CO<jats:sub>2</jats:sub> for over five months, far exceeding other inorganic‐biological hybrid system that can only sustain for hours or days. Biochemical, electrochemical and transcriptomic analyses reveal that the efficient electron uptake of <jats:italic>S. oneidensis</jats:italic> MR‐1 from illuminated CdS nanoparticles supplies sufficient energy to stimulate the previously overlooked reductive glycine pathway for CO<jats:sub>2</jats:sub> fixation. The continuous solar‐to‐chemical conversion is achieved by photon induced electric recycling in sulfur species. Overall, our findings demonstrate that this mineral‐assisted photosynthesis, as a widely existing and unique model of light energy conversion, could support the sustained photoautotrophic growth of non‐photosynthetic microorganisms in nutrient‐lean environments and mediate the reversal of coupled carbon and sulfur cycling, consequently resulting in previously unknown environmental effects. In addition, the hybrid system provides a sustainable and flexible platform to develop a variety of solar products for carbon neutrality.</jats:p>},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Schuman2024,

title = {Integrated Proteomics and Metabolomics Reveal Altered Metabolic Regulation of \textit{Xanthobacter autotrophicus} under Electrochemical Water-Splitting Conditions},

author = {Zachary Schuman and Yongchao Xie and Samantha O’Keeffe and Xun Guan and Jihui Sha and Jingwen Sun and James A. Wohlschlegel and Junyoung O. Park and Chong Liu},

doi = {10.1021/acsami.4c07363},

issn = {1944-8252},

year  = {2024},

date = {2024-08-07},

urldate = {2024-08-07},

journal = {ACS Appl. Mater. Interfaces},

volume = {16},

number = {31},

pages = {40973--40979},

publisher = {American Chemical Society (ACS)},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Guan2024b,

title = {Redox and Energy Homeostasis Enabled by Photocatalytic Material–Microbial Interfaces},

author = {Xun Guan and Sevcan Erşan and Yongchao Xie and Junyoung Park and Chong Liu},

doi = {10.1021/acsnano.4c05763},

issn = {1936-086X},

year  = {2024},

date = {2024-08-06},

urldate = {2024-08-06},

journal = {ACS Nano},

volume = {18},

number = {31},

pages = {20567--20575},

publisher = {American Chemical Society (ACS)},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Che2024,

title = {Synergistic material–microbe interface toward deeper anaerobic defluorination},

author = {Shun Che and Xun Guan and Roselyn Rodrigues and Yaochun Yu and Yongchao Xie and Chong Liu and Yujie Men},

doi = {10.1073/pnas.2400525121},

issn = {1091-6490},

year  = {2024},

date = {2024-07-30},

urldate = {2024-07-30},

journal = {Proc. Natl. Acad. Sci. U.S.A.},

volume = {121},

number = {31},

publisher = {Proceedings of the National Academy of Sciences},

abstract = {<jats:p>

            Per- and polyfluoroalkyl substances (PFAS), particularly the perfluorinated ones, are recalcitrant to biodegradation. By integrating an enrichment culture of reductive defluorination with biocompatible electrodes for the electrochemical process, a deeper defluorination of a C

            <jats:sub>6</jats:sub>

            -perfluorinated unsaturated PFAS was achieved compared to the biological or electrochemical system alone. Two synergies in the bioelectrochemical system were identified: i) The in-series microbial-electrochemical defluorination and ii) the electrochemically enabled microbial defluorination of intermediates. These synergies at the material–microbe interfaces surpassed the limitation of microbial defluorination and further turned the biotransformation end products into less fluorinated products, which could be less toxic and more biodegradable in the environment. This material–microbe hybrid system brings opportunities in the bioremediation of PFAS driven by renewable electricity and warrants future research on mechanistic understanding of defluorinating and electroactive microorganisms at the material–microbe interface for system optimizations.

          </jats:p>},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Guan2024,

title = {Performance evaluation and multidisciplinary analysis of catalytic fixation reactions by material–microbe hybrids},

author = {Xun Guan and Yongchao Xie and Chong Liu},

doi = {10.1038/s41929-024-01151-2},

issn = {2520-1158},

year  = {2024},

date = {2024-05-00},

urldate = {2024-05-00},

journal = {Nat Catal},

volume = {7},

number = {5},

pages = {475--482},

publisher = {Springer Science and Business Media LLC},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Xie2023,

title = {Unexpected metabolic rewiring of CO            _{2}            fixation in H            _{2}            -mediated materials–biology hybrids},

author = {Yongchao Xie and Sevcan Erşan and Xun Guan and Jingyu Wang and Jihui Sha and Shuangning Xu and James A. Wohlschlegel and Junyoung O. Park and Chong Liu},

doi = {10.1073/pnas.2308373120},

issn = {1091-6490},

year  = {2023},

date = {2023-10-17},

urldate = {2023-10-17},

journal = {Proc. Natl. Acad. Sci. U.S.A.},

volume = {120},

number = {42},

publisher = {Proceedings of the National Academy of Sciences},

abstract = {<jats:p>

            A hybrid approach combining water-splitting electrochemistry and H

            <jats:sub>2</jats:sub>

            -oxidizing, CO

            <jats:sub>2</jats:sub>

            -fixing microorganisms offers a viable solution for producing value-added chemicals from sunlight, water, and air. The classic wisdom without thorough examination to date assumes that the electrochemistry in such a H

            <jats:sub>2</jats:sub>

            -mediated process is innocent of altering microbial behavior. Here, we report unexpected metabolic rewiring induced by water-splitting electrochemistry in H

            <jats:sub>2</jats:sub>

            -oxidizing acetogenic bacterium

            <jats:italic>Sporomusa ovata</jats:italic>

            that challenges such a classic view. We found that the planktonic

            <jats:italic>S. ovata</jats:italic>

            is more efficient in utilizing reducing equivalent for ATP generation in the materials–biology hybrids than cells grown with H

            <jats:sub>2</jats:sub>

            supply, supported by our metabolomic and proteomic studies. The efficiency of utilizing reducing equivalents and fixing CO

            <jats:sub>2</jats:sub>

            into acetate has increased from less than 80% of chemoautotrophy to more than 95% under electroautotrophic conditions. These observations unravel previously underappreciated materials’ impact on microbial metabolism in seemingly simply H

            <jats:sub>2</jats:sub>

            -mediated charge transfer between biotic and abiotic components. Such a deeper understanding of the materials–biology interface will foster advanced design of hybrid systems for sustainable chemical transformation.

          </jats:p>},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Guan2022,

title = {Maximizing light-driven CO2 and N2 fixation efficiency in quantum dot–bacteria hybrids},

author = {Xun Guan and Sevcan Erşan and Xiangchen Hu and Timothy L. Atallah and Yongchao Xie and Shengtao Lu and Bocheng Cao and Jingwen Sun and Ke Wu and Yu Huang and Xiangfeng Duan and Justin R. Caram and Yi Yu and Junyoung O. Park and Chong Liu},

doi = {10.1038/s41929-022-00867-3},

issn = {2520-1158},

year  = {2022},

date = {2022-11-00},

urldate = {2022-11-00},

journal = {Nat Catal},

volume = {5},

number = {11},

pages = {1019--1029},

publisher = {Springer Science and Business Media LLC},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Sheng2021,

title = {De Novo Approach to Encapsulating Biocatalysts into Synthetic Matrixes: From Enzymes to Microbial Electrocatalysts},

author = {Tianran Sheng and Xun Guan and Chong Liu and Yude Su},

doi = {10.1021/acsami.1c09708},

issn = {1944-8252},

year  = {2021},

date = {2021-11-10},

urldate = {2021-11-10},

journal = {ACS Appl. Mater. Interfaces},

volume = {13},

number = {44},

pages = {52234--52249},

publisher = {American Chemical Society (ACS)},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Lu2021c,

title = {Perfluorocarbon nanoemulsions create a beneficial O2 microenvironment in N2-fixing biological | inorganic hybrid},

author = {Shengtao Lu and Roselyn M. Rodrigues and Shuyuan Huang and Daniel A. Estabrook and John O. Chapman and Xun Guan and Ellen M. Sletten and Chong Liu},

doi = {10.1016/j.checat.2021.06.002},

issn = {2667-1093},

year  = {2021},

date = {2021-08-00},

urldate = {2021-08-00},

journal = {Chem Catalysis},

volume = {1},

number = {3},

pages = {704--720},

publisher = {Elsevier BV},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{<LineBreak>doi:10.1126/science.abf3427,

title = {Silver nanoparticles boost charge-extraction efficiency in \textit{Shewanella} microbial fuel cells},

author = {Bocheng Cao and Zipeng Zhao and Lele Peng and Hui-Ying Shiu and Mengning Ding and Frank Song and Xun Guan and Calvin K. Lee and Jin Huang and Dan Zhu and Xiaoyang Fu and Gerard C. L. Wong and Chong Liu and Kenneth Nealson and Paul S. Weiss and Xiangfeng Duan and Yu Huang},

url = {https://www.science.org/doi/abs/10.1126/science.abf3427},

doi = {10.1126/science.abf3427},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Science},

volume = {373},

number = {6561},

pages = {1336-1340},

abstract = {The bacterium Shewanella oneidensis is well known to use extracellular electron sinks, metal oxides and ions in nature or electrodes when cultured in a fuel cell, to power the catabolism of organic material. However, the power density of microbial fuel cells has been limited by various factors that are mostly related to connecting the microbes to the anode. Cao et al. found that a reduced graphene oxide–silver nanoparticle anode circumvents some of these issues, providing a substantial increase in current and power density (see the Perspective by Gaffney and Minteer). Electron microscopy revealed silver nanoparticles embedded or attached to the outer cell membrane, possibly facilitating electron transfer from internal electron carriers to the anode. —MAF A silver nanoparticle anode greatly boosts the performance of Shewanella biofilm–based microbial fuel cells. Microbial fuel cells (MFCs) can directly convert the chemical energy stored in organic matter to electricity and are of considerable interest for power generation and wastewater treatment. However, the current MFCs typically exhibit unsatisfactorily low power densities that are largely limited by the sluggish transmembrane and extracellular electron-transfer processes. Here, we report a rational strategy to boost the charge-extraction efficiency in Shewanella MFCs substantially by introducing transmembrane and outer-membrane silver nanoparticles. The resulting Shewanella-silver MFCs deliver a maximum current density of 3.85 milliamperes per square centimeter, power density of 0.66 milliwatts per square centimeter, and single-cell turnover frequency of 8.6 × 105 per second, which are all considerably higher than those of the best MFCs reported to date. Additionally, the hybrid MFCs feature an excellent fuel-utilization efficiency, with a coulombic efficiency of 81%.},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Lu2020,

title = {Electricity-powered artificial root nodule},

author = {Shengtao Lu and Xun Guan and Chong Liu},

doi = {10.1038/s41467-020-15314-9},

issn = {2041-1723},

year  = {2020},

date = {2020-12-00},

urldate = {2020-12-00},

journal = {Nat Commun},

volume = {11},

number = {1},

publisher = {Springer Science and Business Media LLC},

abstract = {<jats:title>Abstract</jats:title><jats:p>Root nodules are agricultural-important symbiotic plant-microbe composites in which microorganisms receive energy from plants and reduce dinitrogen (N<jats:sub>2</jats:sub>) into fertilizers. Mimicking root nodules using artificial devices can enable renewable energy-driven fertilizer production. This task is challenging due to the necessity of a microscopic dioxygen (O<jats:sub>2</jats:sub>) concentration gradient, which reconciles anaerobic N<jats:sub>2</jats:sub> fixation with O<jats:sub>2</jats:sub>-rich atmosphere. Here we report our designed electricity-powered biological|inorganic hybrid system that possesses the function of root nodules. We construct silicon-based microwire array electrodes and replicate the O<jats:sub>2</jats:sub> gradient of root nodules in the array. The wire array compatibly accommodates N<jats:sub>2</jats:sub>-fixing symbiotic bacteria, which receive energy and reducing equivalents from inorganic catalysts on microwires, and fix N<jats:sub>2</jats:sub> in the air into biomass and free ammonia. A N<jats:sub>2</jats:sub> reduction rate up to 6.5 mg N<jats:sub>2</jats:sub> per gram dry biomass per hour is observed in the device, about two orders of magnitude higher than the natural counterparts.</jats:p>},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}

@article{Rodrigues2019,

title = {Perfluorocarbon nanoemulsion promotes the delivery of reducing equivalents for electricity-driven microbial CO2 reduction},

author = {Roselyn M. Rodrigues and Xun Guan and Jesus A. Iñiguez and Daniel A. Estabrook and John O. Chapman and Shuyuan Huang and Ellen M. Sletten and Chong Liu},

doi = {10.1038/s41929-019-0264-0},

issn = {2520-1158},

year  = {2019},

date = {2019-05-00},

urldate = {2019-05-00},

journal = {Nat Catal},

volume = {2},

number = {5},

pages = {407--414},

publisher = {Springer Science and Business Media LLC},

keywords = {Material-Biology},

pubstate = {published},

tppubtype = {article}

}